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secondary antibody conjugated fluorescent alexafluor® dye  (Thermo Fisher)


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    Structured Review

    Thermo Fisher secondary antibody conjugated fluorescent alexafluor® dye
    Secondary Antibody Conjugated Fluorescent Alexafluor® Dye, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+alexafluor+dye/ppr0936818-121-12-15
    Average 90 stars, based on 1 article reviews
    secondary antibody conjugated fluorescent alexafluor® dye - by Bioz Stars, 2026-10
    90/100 stars

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    Incubation:

    Article Title: Anillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence
    Article Snippet: After washing in PBS, cells were permeabilized for 10 minutes in PBS with 0.5% Triton, blocked for 10 minutes in blocking buffer (PBS with 2% BSA, 1.5% goat serum and 0.5% Triton), and incubated for 2 hours with primary antibody specific for ANLN (Sigma, HPA050556, 1:250), B23 (Abcam, ab10530, 1:100) or LMNA/C (Cell Signaling, mAb#4777, 1:2000) dissolved in blocking buffer. .. Then, cells were washed and incubated for 1 hour with a secondary antibody conjugated with fluorescent AlexaFluor ® dye (ThermoFisher Scientific, A-11008 or A-11029, 1:500) dissolved in PBS. ..

    Article Title: nillin Recedes in p53-Dependent Senescence of Tumor Cells and Reappears in Cells Escaping from Senescence
    Article Snippet: After washing in PBS, cells were permeabilized for 10 minutes in PBS with 0.5% Triton, blocked for 10 minutes in blocking buffer (PBS with 2% BSA, 1.5% goat serum and 0.5% Triton), and incubated for 2 hours with primary antibody specific for ANLN (Sigma, HPA050556, 1:250), B23 (Abcam, ab10530, 1:100) or LMNA/C (Cell Signaling, mAb#4777, 1:2000) dissolved in blocking buffer. .. Then, cells were washed and incubated for 1 hour with a secondary antibody conjugated with fluorescent AlexaFluor ® dye (ThermoFisher Scientific, A-11008 or A-11029, 1:500) dissolved in PBS. ..



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